Matrices listed below were spiked with certain level of PI3Kca and the recovery rates were calculated by comparing the measured value to the expected amount of PI3Kca in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-101
95
EDTA plasma(n=5)
95-101
98
heparin plasma(n=5)
92-100
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PI3Kca and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-102%
86-98%
91-104%
EDTA plasma(n=5)
84-101%
83-101%
82-101%
heparin plasma(n=5)
82-98%
82-88%
84-93%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
PI3Kca ELISA Kit allows for the in vitro quantitative determination of PI3Kca concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat PI3Kca(PI3K-catalitic subunit alpha) ELISA Kit