Rat Phenylethanolamine N-methyltransferase

Mfr.No.

E2193Ra

Sensitivity

9.77ng/L

Detection Range

20-4500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Noradrenaline N-methyltransferase;Phenylethanolamine N-methyltransferase;PNMT;PNMTase

Molecular Weight

31670

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2193Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	473.3	33.29	7.03
Intra-Assay	2	18	1168.55	35.16	3.01
Intra-Assay	3	18	685.89	36.34	5.3

Background

Phenylethanolamine N-methyltransferase (PNMT) is an enzyme found primarily in the adrenal medulla that converts norepinephrine (noradrenaline) to epinephrine (adrenaline). It is also expressed in small groups of neurons in the human brain and in selected populations of cardiomyocytes.

Protocol

These standard curves of E2193Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.195	2.281	2.252
	2.367
1200ng/L	1.39	1.338	1.309
	1.286
600ng/L	0.831	0.732	0.704
	0.634
300ng/L	0.414	0.438	0.409
	0.462
150ng/L	0.198	0.186	0.157
	0.173
0ng/L	0.03	0.029	0
	0.028

Gene ID	24661
UniProt ID	P10937

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113325	E2193Ra	48T	$320		Add to Cart
BHE12113325	E2193Ra	96T	$458		Add to Cart

Rat Phenylethanolamine N-methyltransferase - PNMT ELISA Kit

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