Rat Pfkl(6-phosphofructokinase, liver type) ELISA Kit

Mfr.No.

ER0415

Alternative Names

Pfkl ELISA Kit, Phosphohexokinase ELISA Kit, Phosphofructo-1-kinase isozyme B ELISA Kit, PFK-B ELISA Kit, Phosphofructokinase 1 ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Pfkl and the recovery rates were calculated by comparing the measured value to the expected amount of Pfkl in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	94-105	98
EDTA plasma(n=5)	88-102	93
heparin plasma(n=5)	86-97	91

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pfkl and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	86-101%	85-104%	85-103%
EDTA plasma(n=5)	84-95%	84-100%	82-94%
heparin plasma(n=5)	87-98%	84-94%	86-91%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Pfkl/Phosphohexokinase/Phosphofructo-1-kinase isozyme B(PFK-B)/Phosphofructokinase 1/

Notes

For Research Use Only

UniProt ID	P30835
Antigen	PFKL

Applications	ELISA
Applications Notes	Pfkl ELISA Kit allows for the in vitro quantitative determination of Pfkl concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10801262	ER0415	96T	$400		Add to Cart

Rat Pfkl(6-phosphofructokinase, liver type) ELISA Kit

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