Rat peroxisome proliferators activator receptors alpha,PPAR-alpha ELISA Kit

Rat peroxisome proliferators activator receptors alpha,PPAR-alpha ELISA Kit

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Mfr.No.

CSB-E09755r

Alternative Names

peroxisome proliferator-activated receptor alpha, MGC2237, MGC2452, NR1C1, PPAR, PPARalpha, hPPAR, OTTHUMP00000197740, OTTHUMP00000197741, peroxisome proliferative activated receptor, alpha

Reactivity

Rat

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat glycosaminoglycan spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	92	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat glycosaminoglycan in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-97
1:200	Average %	98
	Range %	93-104
1:400	Average %	85
	Range %	81-88
1:800	Average %	88
	Range %	83-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
100	0.081	0.086	0.084
50	0.195	0.201	0.198
25	0.490	0.510	0.500
12.5	0.925	0.902	0.914
6.25	1.688	1.705	1.697
0	2.587	2.612	2.600

Species

Rattus norvegicus

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	P37230
Antigen	peroxisome proliferator-activated receptor alpha

Applications

ELISA

Research Area

PPARA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508477	CSB-E09755r	96T	$495	Add to Cart
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BHE10508477	CSB-E09755r	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.