Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Pdha1 and the recovery rates were calculated by comparing the measured value to the expected amount of Pdha1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-95
92
EDTA plasma(n=5)
89-105
96
heparin plasma(n=5)
87-99
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pdha1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Pdha1 ELISA Kit allows for the in vitro quantitative determination of Pdha1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Pdha1 (Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) ELISA Kit