Rat PDGF-BB ELISA Kit PicoKine®

Mfr.No.

EK1698

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.026	0.045	0.129	0.198	0.478	0.991	1.517	2.024

Description

Rat PDGF-BB ELISA Kit PicoKine® (96 Tests). Quantitate Rat PDGF-BB in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cysteine and glycine-rich protein 2; Cysteine-rich protein 2; CRP2; Smooth muscle cell LIM protein; SmLIM; Csrp2; Smlim

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S82-T190

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat PDGF-BB

Subcellular Localization

Nucleus.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	108	651	1071	121	714	1110
Standard deviation	4.43	33.85	58.9	7.6	52.84	86.58
CV (%)	4.1%	5.2%	5.5%	6.3%	7.4%	7.8%

Protein Function

Drastically down-regulated in response to PDGF-BB or cell injury, that promote smooth muscle cell proliferation and dedifferentiation. Seems to play a role in the development of the embryonic vascular system.

Background

PDGF-BB encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alteatively spliced transcript variants encoding different isoforms have been identified for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	108	107	108	107	107	0.50	0.4%
Sample . 2	651	715	730	643	684	38.22	5.5%%
Sample 3	1071	1047	1054	1145	1079	38.95	3.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	24628
Gene Name	PDGF-BB
UniProt ID	Q05028

Applications	ELISA
Research Area	Growth Factors,Growth Factors/Hormones,Invasion/Microenvironment,Metabolism,Metabolism Processes,Organogenesis,Pathways and Processes,Response To Hypoxia,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001364	EK1698	96 wells/kit	$499		Add to Cart

Rat PDGF-BB ELISA Kit PicoKine®

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