Rat Pde4b(cAMP-specific 3',5'-cyclic phosphodiesterase 4B) ELISA Kit

Mfr.No.

ER0300

Alternative Names

Pde4b ELISA Kit, DPDE4 ELISA Kit, PDE32 ELISA Kit, cAMP-specific 3' ELISA Kit, 5'-cyclic phosphodiesterase 4B ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

78.125-5000pg/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Pde4b and the recovery rates were calculated by comparing the measured value to the expected amount of Pde4b in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	89-105	99
EDTA plasma(n=5)	88-105	94
heparin plasma(n=5)	89-103	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pde4b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	86-100%	86-104%	86-103%
EDTA plasma(n=5)	87-97%	85-101%	82-100%
heparin plasma(n=5)	83-96%	81-95%	91-100%

Sensitivity

< 46.875pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Pde4b//DPDE4/PDE32/cAMP-specific 3',5'-cyclic phosphodiesterase 4B

Notes

For Research Use Only

UniProt ID	P14646
Antigen	PDE4B

Applications	ELISA
Applications Notes	Pde4b ELISA Kit allows for the in vitro quantitative determination of Pde4b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10802939	ER0300	96T	$400		Add to Cart

Rat Pde4b (cAMP-specific 3 ,5 -cyclic phosphodiesterase 4B) ELISA Kit

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