Rat Paraoxonase-1 - PON1 ELISA Kit

Mfr.No.

E0867Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Paraoxonase-1 (also known as PON1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

A-esterase 1;Aromatic esterase 1;PON 1;PON1;Serum aryldialkylphosphatase 1;Serum paraoxonase/arylesterase 1

Reactivity

Rat

Detection Range

1-400ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

39358

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.53ng/ml

Background

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.

Gene ID	84024
UniProt ID	P55159
Antigen	PON1

Research Area

Cardiovascular

Protocol

These standard curves of E0867Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.296	2.238	2.185
	2.18
120ng/ml	1.891	1.862	1.81
	1.834
60ng/ml	1.098	1.181	1.128
	1.263
30ng/ml	0.601	0.612	0.559
	0.623
15ng/ml	0.355	0.398	0.345
	0.441
0ng/ml	0.042	0.053	0
	0.063

Precision

We measured random samples of E0867Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	114	6.28	5.5
Intra-Assay	2	18	38.3	1.78	4.6
Intra-Assay	3	18	33.3	1.86	5.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112118	E0867Ra	48T	$320		Add to Cart
BHE12112118	E0867Ra	96T	$458		Add to Cart

Rat Paraoxonase-1 - PON1 ELISA Kit

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