Rat Pancreatic Triacylglycerol Lipase

Mfr.No.

E1123Ra

Sensitivity

0.25ng/ml

Detection Range

0.5-100ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Pancreatic Triacylglycerol Lipase (also known as PNLIP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Pancreatic lipase;Pancreatic triacylglycerol lipase;PL;PNLIP

Molecular Weight

51440

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1123Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	11.1	0.5	4.5
Intra-Assay	2	18	34.2	1.36	4
Intra-Assay	3	18	14.5	0.79	5.5

Background

Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.

Protocol

These standard curves of E1123Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.172	2.173	2.119
	2.173
32ng/ml	1.446	1.484	1.43
	1.522
16ng/ml	0.733	0.779	0.725
	0.824
8ng/ml	0.427	0.458	0.404
	0.49
4ng/ml	0.248	0.256	0.202
	0.264
0ng/ml	0.044	0.054	0
	0.063

Gene ID	25702
UniProt ID	P27657

Research Area	Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112370	E1123Ra	48T	$320		Add to Cart
BHE12112370	E1123Ra	96T	$458		Add to Cart

Rat Pancreatic Triacylglycerol Lipase - PNLIP ELISA Kit

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