Matrices listed below were spiked with certain level of PACAP-27 and the recovery rates were calculated by comparing the measured value to the expected amount of PACAP-27 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-104
96
EDTA plasma(n=5)
86-96
93
heparin plasma(n=5)
91-100
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PACAP-27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PACAP-27 ELISA Kit allows for the in vitro quantitative determination of PACAP-27 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat PACAP-27(Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit