Rat P-Selectin ELISA kit

Rat P-Selectin ELISA kit

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Mfr.No.

CSB-E07399r

Sensitivity

2.34 ng/mL

Detection Range

9.38 ng/mL-600 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

selectin P (granule membrane protein 140kDa, antigen CD62), RP1-86F14.2, CD62, CD62P, FLJ45155, GMP140, GRMP, LECAM3, PADGEM, PSEL, OTTHUMP00000032609, granulocyte membrane protein, leukocyte-endothelial cell adhesion molecule 3, platelet alpha-granule membrane

Recovery

The recovery of mouse RSPO3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-100
EDTA plasma (n=4)	93	90-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse RSPO3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-94
1:2	Average %	98
	Range %	93-103
1:4	Average %	102
	Range %	98-105
1:8	Average %	94
	Range %	92-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.693	2.800	2.747	2.597
150	1.811	1.970	1.891	1.741
75	1.073	1.135	1.104	0.954
37.5	0.652	0.701	0.677	0.527
18.75	0.369	0.375	0.372	0.222
9.4	0.247	0.266	0.257	0.107
4.7	0.202	0.214	0.208	0.058
0	0.158	0.142	0.150

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P98106

Applications	ELISA
Research Area	SELP

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10508565	CSB-E07399r	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.