Rat p-JNK(Phosphorylated c-Jun N-terminal kinases) ELISA Kit

Mfr.No.

ELK0960

Alternative Names

Bsk; BSK; CG5680; D-JNK; D-junk; DBSK/JNK; dJnk; dJNK; DJNK; DJNK/bsk; DmelCG5680; DmJNK; dNJNK; jnk; Jnk; JNK; jnk (bsk); JNK/SAPK; Junk; p-JNK; P-JNK; pJNK; SAPKa

Reactivity

Rat

Detection Range

0.32-20 ng/mL

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant p-JNK and the recovery rates were calculated by comparing the measured value to the expected amount of p-JNK in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	92-107%	99%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of p-JNK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	95-105%	88-102%	93-101%	83-94%
EDTA plasma(n=5)	92-105%	85-102%	98-105%	88-97%
Heparin plasma(n=5)	85-98%	93-102%	89-97%	85-94%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
20.00	2.201	2.114
10.00	1.638	1.551
5.00	1.182	1.095
2.50	0.921	0.834
1.25	0.532	0.445
0.63	0.369	0.282
0.32	0.216	0.129
0.00	0.087	0.000

Sensitivity

0.146 ng/mL

Limit Of Detection

20 ng/mL

Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-JNK in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Enzyme & Kinase;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15208281	ELK0960	48T	$320		Add to Cart
BHE15208281	ELK0960	96T	$458		Add to Cart

Rat p-JNK(Phosphorylated c-Jun N-terminal kinases) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat p-JNK(Phosphorylated c-Jun N-terminal kinases) ELISA Kit

Rate it now..!!