Rat Osteopontin,OPN ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08393r

Reactivity

Rat

Detection Range

0.06 ng/ml -50 ng/ml

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human VCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	103	97-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human VCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	89-99
1:2	Average %	101
	Range %	96-104
1:4	Average %	91
	Range %	88-93
1:8	Average %	92
	Range %	86-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

0.06 ng/ml

Limit Of Detection

450 nm

UniProt ID	P08721
Antigen	secreted phosphoprotein 1

Applications

ELISA

Research Area

SPP1

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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