Rat Orexin A ELISA Kit

Rat Orexin A ELISA Kit

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Mfr.No.

CSB-E08860r

Alternative Names

Orexin A

Reactivity

Rat

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human ADRBK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	99	93-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ADRBK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-94
1:2	Average %	95
	Range %	90-101
1:4	Average %	97
	Range %	94-102
1:8	Average %	94
	Range %	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.036	2.166	2.101	1.927
600	1.373	1.405	1.389	1.215
300	0.997	0.957	0.977	0.803
150	0.633	0.675	0.654	0.480
75	0.392	0.402	0.397	0.223
37.5	0.294	0.284	0.289	0.115
18.75	0.241	0.233	0.237	0.063
0	0.167	0.180	0.174

Species

Rattus norvegicus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	O55232
Antigen	Orexin A

Applications

ELISA

Research Area

Orexin A

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Orexin A-mediated AKT signaling in the dentate gyrus contributes to the acquisition, expression and reinstatement of morphine-induced conditioned place preference Guo SJ et al,Addict Biol,2015 Mar 10.

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SKU	Supplier No.	Size	Your price
BHE10508447	CSB-E08860r	96T	$856	Add to Cart
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.