Rat OPN(Osteopontin) ELISA Kit

Mfr.No.

ER1214

Alternative Names

OPN ELISA Kit, Osteopontin ELISA Kit, Eta-1 ELISA Kit, Spp1 ELISA Kit, BNSP ELISA Kit, Bone sialoprotein 1 ELISA Kit, MGC110940 ELISA Kit, Nephropontin ELISA Kit, secreted phosphoprotein-1 ELISA Kit, osteopontin ELISA Kit, bone sialoprotein ELISA Kit, SPP-1 ELISA Kit, SPP1 ELISA Kit, CALPHA1 fusion ELISA Kit, Urinary stone protein ELISA Kit, uropontin ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

39.063-2500pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of OPN and the recovery rates were calculated by comparing the measured value to the expected amount of OPN in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	90-104	97
EDTA plasma(n=5)	89-97	92
heparin plasma(n=5)	88-105	97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OPN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	86-105%	87-102%	88-101%
EDTA plasma(n=5)	83-94%	90-100%	88-95%
heparin plasma(n=5)	87-93%	82-100%	83-98%

Sensitivity

< 23.438pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

OPN(Osteopontin)/Eta-1/Spp1/BNSP/Bone sialoprotein 1/MGC110940/Nephropontin/secreted phosphoprotein-1(osteopontin, bone sialoprotein)/SPP-1/SPP1/CALPHA1 fusion/Urinary stone protein/uropontin

Notes

For Research Use Only

UniProt ID	P08721
Antigen	OPN

Applications	ELISA
Applications Notes	OPN ELISA Kit allows for the in vitro quantitative determination of OPN concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10800202	ER1214	96T	$400		Add to Cart

Rat OPN(Osteopontin) ELISA Kit

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