Rat oncostatin M (OSM) ELISA kit

Mfr.No.

CSB-E07402r

Alternative Names

oncostatin M, MGC20461,

Reactivity

Rat

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	87	83-91

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	85
	Range %	80-90
1:2000	Average %	104
	Range %	100-109
1:4000	Average %	91
	Range %	85-96
1:8000	Average %	96
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q65Z15
Antigen	Oncostatin M

Applications

ELISA

Research Area

OSM

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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