Rat Occludin - OCLN ELISA Kit

Mfr.No.

E2185Ra

Sensitivity

7.37ng/L

Detection Range

15-3000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Occludin;Ocln

Molecular Weight

59186

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2185Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1006.02	40.22	4
Intra-Assay	2	18	389.5	21.15	5.43
Intra-Assay	3	18	273.98	14.53	5.3

Background

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. May be involved in the organization of actin in endothelial cells.

Protocol

These standard curves of E2185Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.139	2.107	2.072
	2.075
800ng/L	1.393	1.356	1.322
	1.319
400ng/L	0.739	0.775	0.741
	0.812
200ng/L	0.517	0.468	0.433
	0.419
100ng/L	0.245	0.248	0.213
	0.25
0ng/L	0.039	0.035	0
	0.03

Gene ID	83497
UniProt ID	Q6P6T5

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113317	E2185Ra	48T	$320		Add to Cart
BHE12113317	E2185Ra	96T	$458		Add to Cart

Rat Occludin - OCLN ELISA Kit

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