Rat Occludin (Ocln) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E17291r

Alternative Names

Occludin (Ocln), tight junction protein occludin TM4 minus, Occludin (Ocln), tight junction protein occludin TM4 minus

Reactivity

Rat

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-102
EDTA plasma (n=4)	96	91-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-99
1:2	Average %	86
	Range %	82-91
1:4	Average %	91
	Range %	86-97
1:8	Average %	94
	Range %	88-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q6P6T5
Antigen	Occludin (OCLN)

Applications

ELISA

Research Area

Ocln

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10508443	CSB-E17291r	96T	$695	Add to Cart
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BHE10508443	CSB-E17291r	10x96T	$6602.50	Add to Cart