Rat Nuclear Respiratory Factor 1

Mfr.No.

E1036Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Nuclear Respiratory Factor 1 (also known as NRF1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Alpha palindromic-binding protein;Alpha-pal;Fragment;Nfe2l1_retired;NRF1;NRF-1;Nuclear respiratory factor 1

Reactivity

Rat

Detection Range

0.2-60ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

5339

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.11ng/ml

Background

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication (By similarity).

Gene ID	312195
UniProt ID	Q62792
Antigen	NRF1

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E1036Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.114	2.1	2.056
	2.086
16ng/ml	1.46	1.421	1.377
	1.382
8ng/ml	0.779	0.849	0.805
	0.92
4ng/ml	0.523	0.517	0.473
	0.512
2ng/ml	0.288	0.27	0.226
	0.253
0ng/ml	0.04	0.044	0
	0.049

Precision

We measured random samples of E1036Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	17.51	0.62	3.53
Intra-Assay	2	18	12.16	0.81	6.64
Intra-Assay	3	18	3.48	0.2	5.88

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112284	E1036Ra	48T	$320		Add to Cart
BHE12112284	E1036Ra	96T	$458		Add to Cart

Rat Nuclear Respiratory Factor 1 - NRF1 ELISA Kit

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