Rat Nuclear factor-kappa B(NF-kappaB) ELISA Kit

Rat Nuclear factor-kappa B(NF-kappaB) ELISA Kit

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Mfr.No.

CSB-E13148r

Sensitivity

0.39 pg/mL

Detection Range

1.56 pg/mL-100 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, p105, p50, DNA binding factor KBF1, NF-kappabeta, nuclear factor NF-kappa-B p50 subunit, nuclear factor kappa-B DNA bi

Recovery

The recovery of human CoQ10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	93	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CoQ10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	96
	Range %	91-101
1:40	Average %	99
	Range %	93-104
1:80	Average %	92
	Range %	87-96
1:160	Average %	90
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
50	0.112	0.103	0.108
25	0.208	0.224	0.216
12.5	0.377	0.397	0.387
6.25	0.783	0.718	0.751
3.12	1.121	1.086	1.104
0	1.951	1.829	1.890

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q63369

Applications	ELISA
Research Area	NFKB1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508438	CSB-E13148r	96T	$790	Add to Cart
BHE10508438	CSB-E13148r	5x96T	$3792	Add to Cart
BHE10508438	CSB-E13148r	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.