Matrices listed below were spiked with certain level of Nt5e and the recovery rates were calculated by comparing the measured value to the expected amount of Nt5e in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
95
EDTA plasma(n=5)
94-105
99
heparin plasma(n=5)
87-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nt5e and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Nt5e ELISA Kit allows for the in vitro quantitative determination of Nt5e concentrations in serum, plasma, tissue homogenates and other biological fluids.