Matrices listed below were spiked with certain level of Nps and the recovery rates were calculated by comparing the measured value to the expected amount of Nps in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-103
97
EDTA plasma(n=5)
88-105
98
heparin plasma(n=5)
85-100
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nps and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Nps ELISA Kit allows for the in vitro quantitative determination of Nps concentrations in serum, plasma, tissue homogenates and other biological fluids.