Matrices listed below were spiked with certain level of Nphs2 and the recovery rates were calculated by comparing the measured value to the expected amount of Nphs2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-101
96
EDTA plasma(n=5)
90-105
100
heparin plasma(n=5)
88-94
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nphs2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-96%
89-102%
89-105%
EDTA plasma(n=5)
86-93%
82-101%
84-99%
heparin plasma(n=5)
82-97%
81-99%
84-97%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
Q8K4G9
Applications
ELISA
Applications Notes
Nphs2 ELISA Kit allows for the in vitro quantitative determination of Nphs2 concentrations in serum, plasma, tissue homogenates and other biological fluids.