Rat NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit

Mfr.No.

ELK1503

Sensitivity

0.62 ng/mL

Detection Range

1.57-100 ng/mL

Reactivity

Rat

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible

Recovery

Matrices listed below were spiked with certain level of recombinant NOS2/iNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS2/iNOS in samples.

Matrix	Recovery range	Average
serum(n=5)	81-97%	89%
EDTA plasma(n=5)	84-97%	90%
Heparin plasma(n=5)	82-94%	88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS2/iNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-104%	84-98%	95-105%	88-102%
EDTA plasma(n=5)	88-103%	83-94%	92-105%	89-97%
Heparin plasma(n=5)	87-102%	85-94%	87-101%	89-99%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
100.00	1.979	1.883
50.00	1.562	1.466
25.00	1.029	0.933
12.50	0.885	0.789
6.25	0.561	0.465
3.13	0.357	0.261
1.57	0.268	0.172
0.00	0.096	0.000

Limit Of Detection

100 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	Q06518

Applications	ELISA
Research Area	Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15205599	ELK1503	48T	$320		Add to Cart
BHE15205599	ELK1503	96T	$458		Add to Cart

Rat NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit

Rate it now..!!