Rat Noradrenaline,NA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07022r

Sensitivity

0.49 pg/mL

Detection Range

0.49 pg/mL-2000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Noradrenaline,NA

Recovery

The recovery of hamster ADIPOQ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of hamster ADIPOQ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	85
	Range %	82-90
1:2000	Average %	93
	Range %	90-96
1:4000	Average %	89
	Range %	84-93
1:8000	Average %	94
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	NA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Influence of Endurance Exercise Overloading Patterns Levels Ventricular Catecholamines After a Bout of Lactate Threshold Test in Male Wistar Rat Ahmad Azad . et al,Asian Journal of Sports Medicine,2015 Dec,6(4) Effect of chrysin on nociception in formalin test and serum levels of noradrenalin and corticosterone in rats Gheibi N et al,Basic Clin Neurosci,2015 Feb 15;8(2):2465-2470 Antidepressant-like effect of evodiamine on chronic unpredictable mild stress rats. Jiang ML.et al,Neurosci Lett.,2015 588:154-8. Antidepressant-like effect of lipid extract of Channa striatus in postpartum model of depression in rats Abdul Shukkoor MS.et al,Evid Based Complement Alternat Med,/ Improvement in baroreflex control of renal sympathetic nerve activity in obese Sprague┠awley rats following immunosuppression Khan SA.et al,Acta Physiol (Oxf). ,Apr 29 Effect of Yulangsan Polysaccharide on the Reinstatement of Morphine-Induced Conditioned Place Preference in Sprague-Dawley Rats Chen C.et al,Neurochem Res,Feb 17 High mobility group box-1 in hypothalamic paraventricular nuclei attenuates sympathetic tone in rats at post-myocardial infarction Pang Li.et al,Cardiology Journal,Octobert 5

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