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Home / ELISA Kit

Rat Nicotinamide phosphoribosyltransferase (NAMPT) ELISA Kit

SKU : BHE13700719

Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt) also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin is an enzyme that in humans is encoded by the PBEF1 gene. This protein is the rate-limiting enzyme in the nNicotinamide adenine dinucleotide (NAD+) salvage pathway that converts nicotinamide to nicotinamide mononucleotide in mammals to enable NAD+ biosynthesis. NAMPT has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis.

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Mfr.No.	KTE100521
Description	This Rat Nicotinamide phosphoribosyltransferase (NAMPT) ELISA Kit employs a two-site sandwich ELISA to quantitate NAMPT.
Alternative Names	NAMPT; 1110035O14Rik; DKFZp666B131; MGC117256; PBEF; PBEF1; VF; VISFATIN; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1
Reactivity	Rat
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Rat Nicotinamide phosphoribosyltransferase microplate Rat Nicotinamide phosphoribosyltransferase standard Rat Nicotinamide phosphoribosyltransferase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Rat Nicotinamide phosphoribosyltransferase (NAMPT) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NAMPT. No significant cross-reactivity or interference between Rat NAMPT and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt) also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin is an enzyme that in humans is encoded by the PBEF1 gene. This protein is the rate-limiting enzyme in the nNicotinamide adenine dinucleotide (NAD+) salvage pathway that converts nicotinamide to nicotinamide mononucleotide in mammals to enable NAD+ biosynthesis. NAMPT has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis.
Notes	This Rat Nicotinamide phosphoribosyltransferase (NAMPT) ELISA Kit employs a two-site sandwich ELISA to quantitate NAMPT in samples. An antibody specific for NAMPT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAMPT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAMPT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAMPT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Antigen	NAMPT
Tag	NAMPT
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Rat Nicotinamide phosphoribosyltransferase (NAMPT) ELISA Kit employs a two-site sandwich ELISA to quantitate NAMPT in samples. An antibody specific for NAMPT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAMPT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAMPT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAMPT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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