Rat NF-kappaB(Nuclear Factor Kappa B) ELISA Kit

Mfr.No.

ER1186

Alternative Names

NF-kappaB ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of NF-kappaB and the recovery rates were calculated by comparing the measured value to the expected amount of NF-kappaB in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	88-103	96
EDTA plasma(n=5)	90-101	97
heparin plasma(n=5)	91-102	96

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-kappaB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	87-104%	86-99%	95-103%
EDTA plasma(n=5)	86-96%	83-98%	83-99%
heparin plasma(n=5)	82-95%	84-96%	86-100%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

NF-κB

Notes

For Research Use Only

Antigen	NF-κB

Applications	ELISA
Applications Notes	NF-kappaB ELISA Kit allows for the in vitro quantitative determination of NF-kappaB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

Reference	Journal: Probiotics and Antimicrobial Proteins Title: Association of Vitamin B12, Lactate Dehydrogenase, and Regulation of NF-κB in the Mitigation of Sodium Arsenite-Induced ROS Generation in Uterine Tissue by Commercially Available Probiotics Journal: Acta Biologica Szegediensis Title: Restoration of uterine redox-balance by methanolic extract of Camellia sinensis in arsenicated rats Journal: Environmental Science and Pollution Research Title: Attenuation of utero-toxicity, metabolic dysfunction and inflammation by soy protein concentrate in rats exposed to fluoridated water: consequence of hyperlipidemia in parallel with hypohomocysteinemia

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SKU	Supplier No.	Size	Your price	Quantity
BHE10806905	ER1186	96T	$400		Add to Cart

Rat NF-kappaB(Nuclear Factor Kappa B) ELISA Kit

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