Rat Nf-Kappa-B Inhibitor Alpha

Mfr.No.

E1817Ra

Sensitivity

5.25ng/L

Detection Range

10-2000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat NF-kappa-B Inhibitor Alpha (also known as NFKBIA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IkappaBalpha;I-kappa-B-alpha;IkB-alpha;NF-kappa-B inhibitor alpha;NFKBIA;RL/IF-1

Molecular Weight

35017

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1817Ra within the same batch/lot to ensure the consistency of the kits' performances.

Background

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (By similarity).

Protocol

These standard curves of E1817Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.372	2.325	2.295
	2.278
600ng/L	1.67	1.664	1.633
	1.657
300ng/L	1.102	1.089	1.059
	1.076
150ng/L	0.783	0.722	0.692
	0.661
75ng/L	0.43	0.444	0.414
	0.458
0ng/L	0.029	0.03	0
	0.032

Gene ID	25493
UniProt ID	Q63746

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113017	E1817Ra	48T	$320		Add to Cart
BHE12113017	E1817Ra	96T	$458		Add to Cart

Rat Nf-Kappa-B Inhibitor Alpha - NFKBIA ELISA Kit

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