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Home / ELISA Kit

Rat Neuropeptide Y (NPY) ELISA Kit

SKU : BHE13701246

Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter found in the brain and autonomic nervous system.NPY is a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases.

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Mfr.No.	KTE101000
Reactivity	Rat
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Rat Neuropeptide Y (NPY) ELISA Kit employs a two-site sandwich ELISA to quantitate NPY.
Alternative Names	NPY; PYY4; OTTHUMP00000201946; OTTHUMP00000201947
Detection Method	Colorimetric
Kit Components	Rat Neuropeptide Y microplate Rat Neuropeptide Y standard Rat Neuropeptide Y detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Rat Neuropeptide Y (NPY) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NPY. No significant cross-reactivity or interference between Rat NPY and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter found in the brain and autonomic nervous system.NPY is a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases.
Notes	This Rat Neuropeptide Y (NPY) ELISA Kit employs a two-site sandwich ELISA to quantitate NPY in samples. An antibody specific for NPY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNPY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPY bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Rat Neuropeptide Y (NPY) ELISA Kit employs a two-site sandwich ELISA to quantitate NPY in samples. An antibody specific for NPY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNPY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPY bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13701246	KTE101000	48 T	$359		Add to Cart
BHE13701246	KTE101000	96 T	$619		Add to Cart
BHE13701246	KTE101000	96 Tx5	$2809		Add to Cart
BHE13701246	KTE101000	96 Tx50	$26209		Add to Cart

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