Rat Neuron-specific enolase,NSE ELISA Kit

Mfr.No.

CSB-E07963r

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

enolase 2 (gamma, neuronal), NSE, 2-phospho-D-glycerate hydrolyase, enolase 2, neural enolase, neuron specific gamma enolase, neurone-specific enolase

Recovery

The recovery of human AIF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	99-106
EDTA plasma (n=4)	96	92-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AIF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	104
	Range %	101-108
1:10	Average %	91
	Range %	88-97
1:20	Average %	96
	Range %	91-104
1:40	Average %	89
	Range %	86-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.532	2.522	2.527	2.347
250	2.088	2.027	2.058	1.878
125	1.369	1.459	1.414	1.234
62.5	0.784	0.779	0.782	0.602
31.2	0.445	0.480	0.463	0.283
15.6	0.287	0.299	0.293	0.113
7.8	0.213	0.230	0.222	0.042
0	0.175	0.185	0.180	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P07323

Applications	ELISA
Research Area	ENO2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508416	CSB-E07963r	96T	$856	Add to Cart
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Rat Neuron-specific enolase,NSE ELISA Kit

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