Rat Neuromodulin(GAP43) ELISA kit

Rat Neuromodulin(GAP43) ELISA kit

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Mfr.No.

CSB-EL009231RA

Alternative Names

growth associated protein 43, B-50, PP46, axonal membrane protein GAP-43, calmodulin-binding protein P-57, nerve growth-related peptide GAP43, neural phosphoprotein B-50, neuromodulin, neuron growth-associated protein 43, protein F1

Reactivity

Rat

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of dog MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	97	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	99
	Range %	92-105
1:2	Average %	88
	Range %	80-96
1:4	Average %	87
	Range %	85-90
1:8	Average %	92
	Range %	88-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
800	2.096	2.076	2.086	1.977
400	1.513	1.605	1.559	1.450
200	1.135	1.118	1.127	1.018
100	0.618	0.655	0.637	0.528
50	0.423	0.409	0.416	0.307
25	0.301	0.278	0.290	0.181
12.5	0.230	0.228	0.229	0.120
0	0.110	0.107	0.109	?

Species

Rattus norvegicus

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	P07936
Antigen	Growth Associated Protein 43

Applications

ELISA

Research Area

GAP43

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508413	CSB-EL009231RA	96T	$695	Add to Cart
BHE10508413	CSB-EL009231RA	5x96T	$3336	Add to Cart
BHE10508413	CSB-EL009231RA	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.