Rat Neuroglobin - NGB ELISA Kit

Mfr.No.

E0474Ra

Sensitivity

2.42pg/ml

Detection Range

5-1000pg/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Vitamin C (also known as VC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Neuroglobin;NGB

Molecular Weight

16981

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0474Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	60.4	3.42	5.7
Intra-Assay	2	18	358.5	12.68	3.5
Intra-Assay	3	18	255	16.53	6.5

Background

Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes (By similarity).

Protocol

These standard curves of E0474Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640pg/ml	2.14	2.233	2.158
	2.327
320pg/ml	1.269	1.3	1.225
	1.331
160pg/ml	0.714	0.798	0.723
	0.883
80pg/ml	0.509	0.476	0.401
	0.444
40pg/ml	0.276	0.291	0.216
	0.307
0pg/ml	0.073	0.075	0
	0.077

Gene ID	85382
UniProt ID	Q99JA8

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111762	E0474Ra	48T	$320		Add to Cart
BHE12111762	E0474Ra	96T	$458		Add to Cart

Rat Neuroglobin - NGB ELISA Kit

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