Rat Neurofilament light polypeptide(NEFL) ELISA kit

Rat Neurofilament light polypeptide(NEFL) ELISA kit

Reviews of Rat Neurofilament light polypeptide(NEFL) ELISA kit

Rate it now..!!

Rate it now..!!

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Mfr.No.

CSB-EL015688RA

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

neurofilament, light polypeptide, CMT1F, CMT2E, FLJ53642, NF-L, NF68, NFL, light molecular weight neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, neurofilament triplet L protein, neurofilament, lig

Recovery

The recovery of human ITGB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	93-105
EDTA plasma (n=4)	87	82-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ITGB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	93
	Range %	86-96
1:10	Average %	89
	Range %	85-94
1:20	Average %	88
	Range %	82-92
1:40	Average %	102
	Range %	98-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.361	2.469	2.415	2.284
2000	2.015	1.924	1.970	1.839
1000	1.332	1.356	1.344	1.213
500	0.741	0.732	0.737	0.606
250	0.402	0.413	0.408	0.277
125	0.244	0.255	0.250	0.119
62.5	0.181	0.188	0.185	0.054
0	0.130	0.132	0.131

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P19527

Applications	ELISA
Research Area	NEFL

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10508409	CSB-EL015688RA	96T	$695	Add to Cart
BHE10508409	CSB-EL015688RA	5x96T	$3336	Add to Cart
BHE10508409	CSB-EL015688RA	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.