Rat Nestin - NES ELISA Kit

Mfr.No.

E1174Ra

Sensitivity

0.022ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Nestin (also known as NES) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

NES;Nestin

Molecular Weight

208797

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1174Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.45	0.3	5.47
Intra-Assay	2	18	12.28	0.42	3.41
Intra-Assay	3	18	2.63	0.19	7.34

Background

Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Protocol

These standard curves of E1174Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.151	2.17	2.122
	2.189
8ng/ml	1.135	1.253	1.204
	1.371
4ng/ml	0.778	0.809	0.76
	0.839
2ng/ml	0.475	0.46	0.411
	0.445
1ng/ml	0.265	0.243	0.194
	0.22
0ng/ml	0.042	0.049	0
	0.055

Gene ID	25491
UniProt ID	P21263

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112421	E1174Ra	48T	$320		Add to Cart
BHE12112421	E1174Ra	96T	$458		Add to Cart

Rat Nestin - NES ELISA Kit

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