Rat Nerve growth factor,NGF ELISA kit

Rat Nerve growth factor,NGF ELISA kit

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Mfr.No.

CSB-E04685r

Sensitivity

0.195 pg/mL

Detection Range

0.78 pg/mL-50 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

nerve growth factor (beta polypeptide), Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB, beta-nerve growth factor, nerve growth factor, beta polypeptide, nerve growth factor, beta subunit

Recovery

The recovery of human RPTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-98
EDTA plasma (n=4)	90	87-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human RPTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-97
1:2	Average %	86
	Range %	80-92
1:4	Average %	96
	Range %	87-102
1:8	Average %	93
	Range %	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.796	2.869	2.833	2.711
20	2.131	1.994	2.063	1.941
10	1.152	1.243	1.198	1.076
5	0.705	0.732	0.719	0.597
2.5	0.343	0.366	0.355	0.233
1.25	0.236	0.224	0.230	0.108
0.625	0.218	0.210	0.214	0.092
0	0.124	0.120	0.122	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P25427

Applications	ELISA
Research Area	NGF

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508393	CSB-E04685r	96T	$615	Add to Cart
BHE10508393	CSB-E04685r	5x96T	$2952	Add to Cart
BHE10508393	CSB-E04685r	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.