Rat Neprilysin(MME) ELISA kit

Rat Neprilysin(MME) ELISA kit

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Mfr.No.

CSB-EL014653RA

Sensitivity

11.75 pg/mL

Detection Range

47 pg/mL-3000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

membrane metallo-endopeptidase, CALLA, CD10, DKFZp686O16152, MGC126681, MGC126707, NEP, atriopeptidase, common acute lymphocytic leukemia antigen, enkephalinase, membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase), m

Recovery

The recovery of human IMPDH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	88	82-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IMPDH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-98
1:2	Average %	97
	Range %	93-102
1:4	Average %	94
	Range %	89-98
1:8	Average %	87
	Range %	82-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.632	2.510	2.571	2.448
750	1.476	1.519	1.498	1.375
375	0.867	0.820	0.844	0.721
187.5	0.473	0.465	0.469	0.346
94	0.305	0.311	0.308	0.185
47	0.258	0.246	0.252	0.129
23.5	0.189	0.185	0.187	0.064
0	0.124	0.122	0.123

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P07861

Applications	ELISA
Research Area	MME

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508392	CSB-EL014653RA	96T	$695	Add to Cart
BHE10508392	CSB-EL014653RA	5x96T	$3336	Add to Cart
BHE10508392	CSB-EL014653RA	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.