Rat NEFL(Neurofilament, Light Polypeptide) ELISA Kit

Mfr.No.

ER1184

Alternative Names

NEFL ELISA Kit, NF-L ELISA Kit, 68 kDa neurofilament protein ELISA Kit, Neurofilament triplet L protein ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

15.625-1000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of NEFL and the recovery rates were calculated by comparing the measured value to the expected amount of NEFL in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	87-105	93
EDTA plasma(n=5)	86-104	94
heparin plasma(n=5)	92-103	99

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEFL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-104%	92-100%	88-101%
EDTA plasma(n=5)	82-100%	83-98%	82-92%
heparin plasma(n=5)	81-91%	84-100%	80-99%

Sensitivity

< 9.375pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

NEFL/NF-L/68 kDa neurofilament protein/Neurofilament triplet L protein

Notes

For Research Use Only

UniProt ID	P19527
Antigen	NEFL

Applications	ELISA
Applications Notes	NEFL ELISA Kit allows for the in vitro quantitative determination of NEFL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10803269	ER1184	96T	$400		Add to Cart

Rat NEFL(Neurofilament, Light Polypeptide) ELISA Kit

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