Rat N-acetyl-D-glucosamine Kinase

Mfr.No.

E2167Ra

Sensitivity

5.34ng/L

Detection Range

10-2000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

GlcNAc kinase;N-acetyl-D-glucosamine kinase;N-acetylglucosamine kinase;NAGK

Molecular Weight

37196

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2167Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	593.2	21.83	3.7
Intra-Assay	2	18	817.5	18.26	2.2
Intra-Assay	3	18	333.2	19.21	5.8

Background

Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity.

Protocol

These standard curves of E2167Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.131	2.055	2.007
	1.979
600ng/L	0.916	1.01	0.962
	1.105
300ng/L	0.537	0.496	0.448
	0.455
150ng/L	0.289	0.3	0.252
	0.311
75ng/L	0.17	0.144	0.096
	0.118
0ng/L	0.051	0.048	0
	0.045

Gene ID	297393
UniProt ID	P81799

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113299	E2167Ra	48T	$320		Add to Cart
BHE12113299	E2167Ra	96T	$458		Add to Cart

Rat N-acetyl-D-glucosamine Kinase - NAGK ELISA Kit

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