Rat N-acetyl-beta-D-glucosaminidase,NAG ELISA Kit

Mfr.No.

CSB-E07443r

Sensitivity

0.39 mIU/mL

Detection Range

1.56 mIU/mL-100 mIU/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

N-acetyl-beta-D-glucosaminidase,NAG, ENC-1AS, N-acetyl-beta-glucosaminidase, hexosaminidase B, Beta-hexosaminidase subunit beta(HEXB)

Recovery

The recovery of bovine ISG15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	93	86-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine ISG15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-102
1:2	Average %	92
	Range %	83-99
1:4	Average %	90
	Range %	85-97
1:8	Average %	95
	Range %	83-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q6AXR4

Applications	ELISA
Research Area	NAG

Storage	Store at 2-8°C. Please refer to protocol.

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