Rat Myosin Light Chain 1,3 - Skeletal Muscle Isoform

Mfr.No.

E1804Ra

Sensitivity

0.13ng/ml

Detection Range

0.3-90ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Myosin Light Chain 1,3, Skeletal Muscle Isoform (also known as MYL1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

MLC1/MLC3;MLC1F/MLC3F;MYL 1;MYL1;Myosin light chain 1/3, skeletal muscle isoform;Myosin light chain A1/A2;Myosin light chain alkali 1/2

Molecular Weight

20680

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1804Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	21.2	1.49	7
Intra-Assay	2	18	28.4	0.99	3.5
Intra-Assay	3	18	8.7	0.67	7.7

Background

Non-regulatory myosin light chain required for proper formation and/or maintenance of myofibers, and thus appropriate muscle function.

Protocol

These standard curves of E1804Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.194	2.177	2.127
	2.16
24ng/ml	1.471	1.369	1.318
	1.266
12ng/ml	0.727	0.794	0.744
	0.861
6ng/ml	0.502	0.529	0.479
	0.556
3ng/ml	0.316	0.301	0.25
	0.286
0ng/ml	0.051	0.05	0
	0.05

Gene ID	56781
UniProt ID	P02600

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113005	E1804Ra	48T	$320		Add to Cart
BHE12113005	E1804Ra	96T	$458		Add to Cart

Rat Myosin Light Chain 1,3 - Skeletal Muscle Isoform - MYL1 ELISA Kit

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