Rat Myeloperoxidase (MPO) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08722r

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

myeloperoxidase,MPO

Recovery

The recovery of bovine ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	98	94-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-97
1:2	Average %	97
	Range %	93-104
1:4	Average %	85
	Range %	82-91
1:8	Average %	90
	Range %	85-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	D3ZGE2

Applications	ELISA
Research Area	MPO

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The protective effects of taurine on experimental acute pancreatitis in a rat model Akay C,Hum Exp Toxicol,2013 Mar 27. Human oral isolate Lactobacillus fermentum AGR1487 induces a pro-inflammatory response in germ-free rat colons. Anderson RC. et al,Sci Rep.,2016 6:20318. Baicalin inhibits toll-like receptor 2/4 expression and downstream signaling in rat experimental periodontitis Sun JY.et al,Int Immunopharmacol.,36:86-93 Protective effect of coptisine free base on indomethacin-induced gastric ulcers in rats: Characterization of potential molecular mechanisms Luo C.et al,Life Sci,Dec 6 The Ameliorative Potential of Dexmedetomidine and Benincasa Cerifera Extract in Renal Ischemia/Reperfusion Injury in a Streptozotocin-Induced Diabetic Model Gehan A Hegazy.et al,Biomedical and Pharmacology,1 April Influence of Lycopene Administration on Neopterin, Myeloperoxidase and gamma Glutamyl Transferase in Diabetic Rats Ahmet Ufuk .et al,FRESEN ENVIRON BULL,June Protective effect of pentoxifylline on oxidative renal cell injury associated with renal crystal formation in a hyperoxaluric rat model Hayrettin Ozturk.et al,Urolithiasis,July 6

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