Rat Myeloperoxidase (MPO) ELISA kit

Rat Myeloperoxidase (MPO) ELISA kit

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Mfr.No.

CSB-E08722r

Alternative Names

myeloperoxidase,MPO

Reactivity

Rat

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of bovine ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	98	94-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-97
1:2	Average %	97
	Range %	93-104
1:4	Average %	85
	Range %	82-91
1:8	Average %	90
	Range %	85-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average
50	0.212	0.207	0.210
25	0.345	0.332	0.339
12.5	0.589	0.607	0.598
6.25	1.077	1.106	1.092
3.125	2.214	2.123	2.169

Species

Rattus norvegicus

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	D3ZGE2
Antigen	myeloperoxidase,MPO

Applications

ELISA

Research Area

MPO

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The protective effects of taurine on experimental acute pancreatitis in a rat model Akay C,Hum Exp Toxicol,2013 Mar 27. Human oral isolate Lactobacillus fermentum AGR1487 induces a pro-inflammatory response in germ-free rat colons. Anderson RC. et al,Sci Rep.,2016 6:20318. Baicalin inhibits toll-like receptor 2/4 expression and downstream signaling in rat experimental periodontitis Sun JY.et al,Int Immunopharmacol.,36:86-93 Protective effect of coptisine free base on indomethacin-induced gastric ulcers in rats: Characterization of potential molecular mechanisms Luo C.et al,Life Sci,Dec 6 The Ameliorative Potential of Dexmedetomidine and Benincasa Cerifera Extract in Renal Ischemia/Reperfusion Injury in a Streptozotocin-Induced Diabetic Model Gehan A Hegazy.et al,Biomedical and Pharmacology,1 April Influence of Lycopene Administration on Neopterin, Myeloperoxidase and gamma Glutamyl Transferase in Diabetic Rats Ahmet Ufuk .et al,FRESEN ENVIRON BULL,June Protective effect of pentoxifylline on oxidative renal cell injury associated with renal crystal formation in a hyperoxaluric rat model Hayrettin Ozturk.et al,Urolithiasis,July 6

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SKU	Supplier No.	Size	Your price
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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.