Rat Muscarinic Acetylcholine Receptor M4

Mfr.No.

E2159Ra

Sensitivity

10.21ng/L

Detection Range

20-4500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CHRM4;M4;Muscarinic acetylcholine receptor M4

Molecular Weight

52921

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2159Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	514.53	34.97	6.8
Intra-Assay	2	18	1276.4	45.08	3.53
Intra-Assay	3	18	1000.97	65.81	6.57

Background

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

Protocol

These standard curves of E2159Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.077	2.194	2.147
	2.31
1200ng/L	1.31	1.323	1.276
	1.335
600ng/L	0.647	0.746	0.699
	0.845
300ng/L	0.447	0.424	0.377
	0.4
150ng/L	0.228	0.236	0.189
	0.244
0ng/L	0.04	0.047	0
	0.054

Gene ID	25111
UniProt ID	P08485

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113291	E2159Ra	48T	$320		Add to Cart
BHE12113291	E2159Ra	96T	$458		Add to Cart

Rat Muscarinic Acetylcholine Receptor M4 - CHRM4 ELISA Kit

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