Rat Muscarinic Acetylcholine Receptor M3

Mfr.No.

E2158Ra

Sensitivity

0.033ng/mL

Detection Range

0.06-18ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CHRM3;Muscarinic acetylcholine receptor M3

Molecular Weight

66066

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2158Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.59	0.26	4.01
Intra-Assay	2	18	2.1	0.11	5.23
Intra-Assay	3	18	3.91	0.19	4.75

Background

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Protocol

These standard curves of E2158Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
9.6ng/mL	2.18	2.184	2.153
	2.188
4.8ng/mL	1.182	1.32	1.289
	1.459
2.4ng/mL	0.765	0.728	0.696
	0.69
1.2ng/mL	0.389	0.41	0.378
	0.431
0.6ng/mL	0.147	0.169	0.138
	0.191
0ng/mL	0.029	0.031	0
	0.034

Gene ID	24260
UniProt ID	P08483

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113290	E2158Ra	48T	$320		Add to Cart
BHE12113290	E2158Ra	96T	$458		Add to Cart

Rat Muscarinic Acetylcholine Receptor M3 - CHRM3 ELISA Kit

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