Rat MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit

Mfr.No.

ELK8233

Sensitivity

0.94 ng/mL

Detection Range

3.13-200 ng/mL

Reactivity

Rat

Assay Duration

Assay Type

Competitive Inhibition

Alternative Names

MOTS-c

Recovery

Matrices listed below were spiked with certain level of recombinant MOTS-c and the recovery rates were calculated by comparing the measured value to the expected amount of MOTS-c in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	78-90%	84%
Heparin plasma(n=5)	89-107%	90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MOTS-c and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-102%	86-92%	91-103%	89-97%
EDTA plasma(n=5)	95-102%	87-101%	82-91%	85-97%
Heparin plasma(n=5)	82-103%	85-96%	92-102%	81-90%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
200.00	0.185
100.00	0.413
50.00	0.596
25.00	0.997
12.50	1.197
6.25	1.522
3.13	1.889
0.00	2.101

Sample Type

Cell lysates, Serum, Cell culture supeatants, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

200 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MOTS-c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MOTS-c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MOTS-c in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Developmental science;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200342	ELK8233	48T	$320		Add to Cart
BHE15200342	ELK8233	96T	$458		Add to Cart

Rat MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit

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