Rat MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit

Mfr.No.

ELK8233

Sensitivity

0.94 ng/mL

Detection Range

3.13-200 ng/mL

Reactivity

Rat

Assay Duration

Assay Type

Competitive Inhibition

Alternative Names

MOTS-c

Recovery

Matrices listed below were spiked with certain level of recombinant MOTS-c and the recovery rates were calculated by comparing the measured value to the expected amount of MOTS-c in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	78-90%	84%
Heparin plasma(n=5)	89-107%	90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MOTS-c and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-102%	86-92%	91-103%	89-97%
EDTA plasma(n=5)	95-102%	87-101%	82-91%	85-97%
Heparin plasma(n=5)	82-103%	85-96%	92-102%	81-90%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
200.00	0.185
100.00	0.413
50.00	0.596
25.00	0.997
12.50	1.197
6.25	1.522
3.13	1.889
0.00	2.101

Limit Of Detection

200 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MOTS-c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MOTS-c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MOTS-c in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Developmental science;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200342	ELK8233	48T	$320		Add to Cart
BHE15200342	ELK8233	96T	$458		Add to Cart

Rat MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit

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