Rat Mothers Against Decapentaplegic Homolog 7

Mfr.No.

E0078Ra

Sensitivity

0.025ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

MAD homolog 7;Mothers against decapentaplegic homolog 7;Mothers against DPP homolog 7;SMAD 7;SMAD family member 7;Smad7

Molecular Weight

46458

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0078Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.1	0.1	4.6
Intra-Assay	2	18	4.2	0.17	4.1
Intra-Assay	3	18	1.4	0.06	4

Background

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Protocol

These standard curves of E0078Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.105	2.054	2.015
	2.003
4ng/ml	1.359	1.439	1.4
	1.519
2ng/ml	0.663	0.666	0.627
	0.669
1ng/ml	0.348	0.386	0.347
	0.423
0.5ng/ml	0.162	0.151	0.112
	0.141
0ng/ml	0.021	0.039	0
	0.057

Gene ID	81516
UniProt ID	O88406

Research Area	Signal Transduction; Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111398	E0078Ra	48T	$320		Add to Cart
BHE12111398	E0078Ra	96T	$458		Add to Cart

Rat Mothers Against Decapentaplegic Homolog 7 - SMAD7 ELISA Kit

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