Rat Mothers Against Decapentaplegic Homolog 5

Mfr.No.

E1690Ra

Sensitivity

5.19ng/L

Detection Range

10-3800ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

MAD homolog 5;Madh5;Mothers against decapentaplegic homolog 5;Mothers against DPP homolog 5;SMAD 5;SMAD family member 5;Smad5

Molecular Weight

52215

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1690Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	282.42	18.03	6.38
Intra-Assay	2	18	783.04	39.84	5.09
Intra-Assay	3	18	1112.49	34.26	3.08

Background

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD) (By similarity).

Protocol

These standard curves of E1690Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.408	2.433	2.38
	2.458
1000ng/L	1.461	1.481	1.428
	1.5
500ng/L	0.712	0.815	0.762
	0.917
250ng/L	0.435	0.474	0.421
	0.513
125ng/L	0.212	0.216	0.163
	0.22
0ng/L	0.04	0.053	0
	0.065

Gene ID	59328
UniProt ID	Q9R1V3

Research Area	Signal Transduction; Epigenetics and Nuclear Signaling; Stem Cells; Developmental Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112896	E1690Ra	48T	$320		Add to Cart
BHE12112896	E1690Ra	96T	$458		Add to Cart

Rat Mothers Against Decapentaplegic Homolog 5 - SMAD5 ELISA Kit

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