Rat Mothers Against Decapentaplegic Homolog 2

Mfr.No.

E1258Ra

Sensitivity

5.11ng/L

Detection Range

10-3000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

MAD homolog 2;Mad-related protein 2;Mothers against decapentaplegic homolog 2;Mothers against DPP homolog 2;SMAD 2;SMAD family member 2;SMAD2

Molecular Weight

52239

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1258Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	571.1	31.82	5.6
Intra-Assay	2	18	951.7	31.78	3.3
Intra-Assay	3	18	325.7	18.87	5.8

Background

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).

Protocol

These standard curves of E1258Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.378	2.421	2.37
	2.463
800ng/L	1.567	1.451	1.4
	1.335
400ng/L	0.809	0.792	0.742
	0.775
200ng/L	0.396	0.387	0.337
	0.379
100ng/L	0.255	0.252	0.202
	0.25
0ng/L	0.041	0.051	0
	0.06

Gene ID	29357
UniProt ID	O70436

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112501	E1258Ra	48T	$320		Add to Cart
BHE12112501	E1258Ra	96T	$458		Add to Cart

Rat Mothers Against Decapentaplegic Homolog 2 - SMAD2 ELISA Kit

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