Rat monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA kit

Mfr.No.

CSB-E07429r

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF, monocyte chemoattractant protein-1, monocyte chemotactic and activating factor, monocyte secretory protein JE, small inducible cytokine

Recovery

The recovery of rat S100A9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	84	80-88

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat S100A9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	100
	Range %	95-106
1:4	Average %	89
	Range %	84-93
1:8	Average %	96
	Range %	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.105	2.035	2.070	1.895
15	1.626	1.526	1.576	1.401
7.5	1.013	1.059	1.036	0.861
3.75	0.674	0.702	0.688	0.513
1.8	0.502	0.517	0.510	0.335
0.9	0.371	0.394	0.383	0.208
0.45	0.255	0.241	0.248	0.073
0	0.172	0.178	0.175	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P14844

Applications	ELISA
Research Area	CCL2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508353	CSB-E07429r	96T	$615	Add to Cart
BHE10508353	CSB-E07429r	5x96T	$2952	Add to Cart
BHE10508353	CSB-E07429r	10x96T	$5842.50	Add to Cart

Rat monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA kit

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