Matrices listed below were spiked with certain level of MLCK/MYLK and the recovery rates were calculated by comparing the measured value to the expected amount of MLCK/MYLK in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-105
94
EDTA plasma(n=5)
87-105
97
heparin plasma(n=5)
86-102
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MLCK/MYLK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-101%
89-97%
91-103%
EDTA plasma(n=5)
86-99%
86-101%
83-97%
heparin plasma(n=5)
86-100%
81-98%
83-95%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P20689
Applications
ELISA
Applications Notes
MLCK/MYLK ELISA Kit allows for the in vitro quantitative determination of MLCK/MYLK concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat MLCK/MYLK (Myosin light chain kinase) ELISA Kit