Rat MIP1a(Macrophage Inflammatory Protein 1 Alpha) ELISA Kit

Mfr.No.

ELK1243

Sensitivity

0.061 ng/mL

Detection Range

0.16-10 ng/mL

Reactivity

Rat

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

MIP1 Alpha; CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein

Recovery

Matrices listed below were spiked with certain level of recombinant MIP1a and the recovery rates were calculated by comparing the measured value to the expected amount of MIP1a in samples.

Matrix	Recovery range	Average
serum(n=5)	82-94%	88%
EDTA plasma(n=5)	82-94%	88%
Heparin plasma(n=5)	85-97%	91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIP1a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	90-99%	95-102%	82-90%
EDTA plasma(n=5)	90-99%	95-106%	87-101%	82-97%
Heparin plasma(n=5)	80-92%	86-102%	87-103%	85-101%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
10.00	2.128	2.061
5.00	1.771	1.704
2.50	1.037	0.970
1.25	0.683	0.616
0.63	0.506	0.439
0.32	0.361	0.294
0.16	0.225	0.158
0.00	0.067	0.000

Sample Type

Cell lysates, Serum, Cell culture supeatants, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

10 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIP1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIP1a in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P50229

Applications	ELISA
Research Area	Cytokine;Infection immunity;

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15205640	ELK1243	48T	$265		Add to Cart
BHE15205640	ELK1243	96T	$376		Add to Cart

Rat MIP1a(Macrophage Inflammatory Protein 1 Alpha) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat MIP1a(Macrophage Inflammatory Protein 1 Alpha) ELISA Kit

Rate it now..!!